Moralo oa Setsebi oa Boleng bo Holimo Protheinease K Nomoro ea CAS 39450-01-6 ka Theko e Molemohali
Hangata re sebetsa re le basebetsi ba bonahalang re etsa bonnete ba hore re u fa boleng bo botle ka ho fetisisa hammoho le theko e ntle ka ho fetisisa ea thekiso bakeng sa Boleng bo Phahameng ba Moralo oa SetsebiProtheine K Nomoro ea CAS 39450-01-6ka Theko e Molemohali, Bakeng sa tlhahisoleseling e eketsehileng le lintlha, ka kopo bua le rona kapele kamoo ho ka khonehang!
Hangata re sebetsa re le basebetsi ba bonahalang re etsa bonnete ba hore re u fa boleng bo botle ka ho fetisisa hammoho le theko e ntle ka ho fetisisa ea thekiso bakeng saLi-albamo tsa Limber le tsa Molek'hule tsa China Tritirachium, Taolo e tiileng ea boleng e etsoa khokahanong e 'ngoe le e 'ngoe ea ts'ebetso eohle ea tlhahiso. Re tšepa ka tieo ho theha tšebelisano-'moho e mosa le e molemo le uena. Re ipapisitse le litharollo tsa boleng bo holimo le tšebeletso e phethahetseng ea pele ho thekiso/kamora thekiso ke mohopolo oa rona, bareki ba bang ba sebelisane le rona ka lilemo tse fetang 5.
| Mokelikeli oa nomoro ea 1 | |
| Mosebetsi o ikhethileng oa protheine> 800U/mL, mahloriso ke 20mg/mL, ha ho na masala a Nickase | |
| Phofo ea nomoro ea 2 | |
| Ponahalo | Phofo e tšoeu e lyophilized |
| Bohloeki ba electrophoretic | ≥95% |
| Mosebetsi oa li-enzyme | ≥30U/mg |
| Masalla a asiti ea nyutlelie | Ha e bonahale |
| Masalla a Deoxyribonuclease | Ha e bonahale |
| Masalla a Ribonuclease | Ha e bonahale |
| Nomoro ea kariki | G1205-10ML |
| Polokelo | Ha e bolokiloe ho -20 °C, sehlahisoa se boloka ts'ebetso bonyane selemo se le seng, se tsamaisoa ka mokotla oa leqhoa. |
Protheine Kke protease ea serine e leng ea lelapa la subtilisin e nang le ts'ebetso e sebetsang ea enzymatic le ho khetheha ha substrate ka bophara, e khethollang ka ho khetheha libonto tsa ester le li-peptide tse haufi le C-terminus ea li-amino acid tse hydrophobic, li-amino acid tse nang le sebabole, le libonto tsa amino acid tse nkhang hamonate, tse atisang ho sebelisoa ho senya liprotheine ho hlahisa li-peptide tse khutšoane.Protheine Ke na le tšobotsi ea triad e tloaelehileng ea catalytic Asp39-His69-Ser224 e ikhethang ho li-protease tsa serine 'me e na le libaka tse peli tsa Ca2+ tse tlamang ho potoloha setsi se sebetsang ho eketsa botsitso ba eona le ho boloka ts'ebetso e phahameng ea enzymatic tlas'a maemo a mangata.
1. Sete ea tlhahlobo ea liphatsa tsa lefutso.
2. Lisebelisoa tsa ho ntša RNA le DNA.
3. Ntša likarolo tse seng tsa protheine liseleng 'me u senye litšila tsa protheine (joalo ka ho lokisetsa liente tsa DNA le heparin).
4. Tokisetso ea DNA ea Chromosomal bakeng sa Pulse Electrophoresis.
5. Ho hlakola ka bophirima.
6. Ntlafatso le tlhahiso ea li-reagent tsa albin tse nang le enzyme glycated lefapheng la tlhahlobo ea in vitro.
1. 30mg/botlolo 1g/botlolo kapa 100g/botlolo
2. 1kgs/mokotlana oa foil
Proteinase K ke protease e tsitsitseng ea serine e nang le ho khetheha ho pharaletseng ha substrate. E senya liprotheine tse ngata sebakeng sa tlhaho esita le ha ho na le sesepa. Ha e kenngoe tšebetsong ke li-ion tsa tšepe, li-chelating agents (mohlala, EDTA), li-reagent tsa sulfhydryl, kapa ke li-tripsin kapa li-inhibitor tsa chymotrypsin. E tsitsitse holim'a pH e pharaletseng (4-12.5), ka ts'ebetso e ntle ka ho fetisisa ho pH 6.5-9.5. Ts'ebetso e ka hlohlelletsoa ka ho eketsa li-denaturing agents (SDS le Urea). Denaturation e potlakileng ea enzyme e etsahala mochesong o kaholimo ho 70°C. Autolysis ea enzyme e etsahala ka bongata ho alkaline pH. Leha ho le joalo, Proteinase K ha e kenngoe tšebetsong ka botlalo ke autolysis. Likaroloana tse ling tsa enzyme li tsoela pele ho boloka ts'ebetso ea tsona e felletseng ea proteolytic, esita le ka mor'a autolysis e kholo. Proteinase K e sebelisoa khafetsa lits'ebetsong tsa baeloji ea limolek'hule ho sila liprotheine tse sa batleheng, joalo ka li-nucleases litokisetsong tsa DNA kapa RNA tse tsoang ho likokoana-hloko, lisele tse lengoang le limela. Hangata enzyme ena e sebediswa ho 50–200 ug/ml ditokisetsong tsa nucleic acid ho pH 7.5–8.0 le 37~55oC. Dinako tsa ho futhumala di fapana ho tloha metsotsong e 30 ho isa ho dihora tse 18.
Protease ea serine e tsitsitseng le e arabelang haholo; e sebelisetsoa ho arola protheine le nucleic acid. Proteinase K e sebelisoa ho hloekiseng RNA le DNA ho tsoa liseleng kapa mela ea sele.
Hangata re sebetsa re le basebetsi ba bonahalang re etsa bonnete ba hore re u fa boleng bo botle ka ho fetisisa hammoho le theko e ntle ka ho fetisisa ea thekiso bakeng sa Professional Design High Quality Proteinase K CAS No. 39450-01-6 ka Theko e Molemohali, Bakeng sa tlhaiso-leseling e eketsehileng le lintlha, ka kopo bua le rona kapele kamoo ho ka khonehang!
Moralo oa SetsebiLi-albamo tsa Limber le tsa Molek'hule tsa China Tritirachium, Taolo e tiileng ea boleng e etsoa khokahanong e 'ngoe le e 'ngoe ea ts'ebetso eohle ea tlhahiso. Re tšepa ka tieo ho theha tšebelisano-'moho e mosa le e molemo le uena. Re ipapisitse le litharollo tsa boleng bo holimo le tšebeletso e phethahetseng ea pele ho thekiso/kamora thekiso ke mohopolo oa rona, bareki ba bang ba sebelisane le rona ka lilemo tse fetang 5.
