fektheri theko e tlaase Alkaline ProteinaseK CAS 39450-01-6
Re ikemiselitse ho fana ka sekhahla sa tlholisano, lihlahisoa tse ikhethang le litharollo tsa boleng bo holimo, joalo ka phepelo e potlakileng bakeng sa theko e tlase ea feme ea Alkaline.ProteinaseK CAS 39450-01-6, Re ntse re batla ka tieo ho sebelisana le bareki hohle lefats'eng lohle. Re dumela hore re tla o kgotsofatsa. Re boetse re amohela bareki ka mofuthu ho ea mokhatlong oa rona le ho reka lihlahisoa tsa rona.
Re ikemiselitse ho fana ka sekhahla sa tlholisano, lihlahisoa tse ikhethang le litharollo tsa boleng bo holimo, joalo ka ho fana ka thepa e potlakileng bakeng saChina Alkaline ProteinaseK le 39450-01-6, Ka lebaka la boleng bo botle le litheko tse utloahalang, thepa ea rona e rometsoe linaheng tse fetang 10 le libaka. Re labalabela ho sebelisana le bareki bohle ba tsoang lapeng le kantle ho naha. Ho feta moo, khotsofalo ea bareki ke sepheo sa rona sa ka ho sa feleng.
| No.1 mokelikeli | |
| Ts'ebetso e khethehileng ea protheine> 800U/mL, khatello ea maikutlo ke 20mg/mL, ha ho na masala a Nickase. | |
| No.2 phofo | |
| Ponahalo | White lyophilized phofo |
| Ho hloeka ha electrophoretic | ≥95% |
| Ts'ebetso ea enzyme | ≥30U/mg |
| Nucleic acid mesaletsa | E sa bonahaleng |
| Masala a Deoxyribonuclease | E sa bonahaleng |
| Masala a ribonuclease | E sa bonahaleng |
| Koloi No. | G1205-10ML |
| Polokelo | Ha e bolokiloe ho -20 ° C, sehlahisoa se boloka ts'ebetso bonyane lilemo tse 1, lipalangoang tsa mokotla oa leqhoa. |
Proteinase Kke serine protease ea lelapa la subtilisin e nang le ts'ebetso e sebetsang ea enzymatic le sebopeho se pharaletseng sa substrate, eo ka ho khetheha e senyang litlamo tsa ester le li-peptide tse haufi le C-terminus ea hydrophobic amino acid, li-amino acid tse nang le sebabole, le litlamo tse nkhang hamonate tsa amino acid. hangata e sebelisoa ho theola liprotheine ho hlahisa li-peptide tse khutšoane. Proteinase K e na le tšobotsi e tloaelehileng ea catalytic triad Asp39-His69-Ser224 e ikhethang ho serine proteases 'me e na le libaka tse peli tse tlamang tsa Ca2+ ho potoloha setsi se sebetsang ho eketsa botsitso le ho boloka ts'ebetso e phahameng ea enzymatic tlas'a maemo a mangata a mangata.
1. Setsi sa tlhahlobo ea liphatsa tsa lefutso.
2. RNA le DNA extraction kits.
3. Ntša likaroloana tse se nang protheine ka har'a lisele le ho senya litšila tsa protheine (joaloka ho lokisa liente tsa DNA le heparin).
4. Ho lokisetsa DNA ea Chromosomal bakeng sa Pulse Electrophoresis.
5. Ho phunya ha Bophirimela.
6. Nts'etsopele le tlhahiso ea li-reagents tsa albin tsa enzymatic glycated tšimong ea tlhahlobo ea in vitro.
1. 30mg/botlolo 1g/botlolo kapa 100g/botlolo
2. 1kgs / mokotla oa foil
Proteinase K ke serine protease e tsitsitseng e nang le likarolo tse pharaletseng tsa substrate. E senya liprotheine tse ngata sebakeng sa matsoalloa esita le ka pel'a li-detergents. Ha e kenngoe ke li-ion tsa tšepe, li-chelating agents (mohlala, EDTA), li-reagents tsa sulfhydryl, kapa ka tripsin kapa chymotrypsin inhibitors. E tsitsitse holim'a mefuta e mengata ea pH (4-12.5), e nang le ts'ebetso e nepahetseng ho pH 6.5-9.5. Ts'ebetso e ka khothaletsoa ka ho eketsoa ha li-agent tsa denaturing (SDS le Urea). Ho phatloha ka potlako ha enzyme ho etsahala mochesong o ka holimo ho 70 ° C. Autolysis ea enzyme e etsahala ka ho eketsehileng ho alkaline pH. Leha ho le joalo, Proteinase K ha e sebetse ka ho feletseng ka autolysis. Likaroloana tse ling tsa enzyme li tsoela pele ho boloka ts'ebetso ea tsona e felletseng ea proteolytic, le kamora ho itlhahloba ho hoholo. Proteinase K e sebelisoa khafetsa lits'ebetsong tsa baeloji ea limolek'hule ho sila liprotheine tse sa batleheng, joalo ka li-nuclease ho DNA kapa litokisetso tsa RNA ho tsoa ho likokoana-hloko, lisele tse tsoetsoeng le limela. Enzyme e atisa ho sebelisoa ho 50-200 ug / ml ka litokisetso tsa nucleic acid ka pH 7.5-8.0 le 37 ~ 55oC. Linako tsa ho incubation li fapana ho tloha metsotso e 30 ho isa ho lihora tse 18.
Serine protease e tsitsitseng le e sebetsang haholo; se sebedisoang bakeng sa protheine le nucleic acid ho itšehla thajana.Proteinase K e sebelisoa ho hloekisoa ha RNA le DNA ho tloha liseleng kapa liseleng.
Re ikemiselitse ho fana ka sekhahla sa tlholisano, lihlahisoa tse ikhethang le litharollo tsa boleng bo holimo, joalo ka phepelo e potlakileng ea theko e tlase ea feme Alkaline ProteinaseK.CAS39450-01-6, Re ntse re batla ka tieo ho sebelisana le bareki hohle lefats'eng lohle. Re dumela hore re tla o kgotsofatsa. Re boetse re amohela bareki ka mofuthu ho ea mokhatlong oa rona le ho reka lihlahisoa tsa rona.
theko e tlase ea femeChina Alkaline ProteinaseK le 39450-01-6, Ka lebaka la boleng bo botle le litheko tse utloahalang, thepa ea rona e rometsoe linaheng tse fetang 10 le libaka. Re labalabela ho sebelisana le bareki bohle ba tsoang lapeng le kantle ho naha. Ho feta moo, khotsofalo ea bareki ke sepheo sa rona sa ka ho sa feleng.
