Theko e tlase ea fektheri Alkaline ProteinaseK CAS 39450-01-6
Re ikemiselitse ho fana ka theko e phahameng ea tlholisano, lihlahisoa le litharollo tse ikhethang, hammoho le phano e potlakileng bakeng sa Alkaline ea fektheri e theko e tlase.ProtheineaseK CAS 39450-01-6Re ntse re labalabela ka tieo ho sebelisana le bareki lefatšeng ka bophara. Re lumela hore re tla u khotsofatsa. Re boetse re amohela bareki ka mofuthu ho ea mokhatlong oa rona le ho reka lihlahisoa tsa rona.
Re ikemiselitse ho fana ka theko e phahameng ea tlholisano, lihlahisoa le litharollo tse ikhethang, hammoho le phano e potlakileng bakeng saProteinase ea Alkaline ea Chaena le 39450-01-6, Ka lebaka la boleng bo botle le litheko tse utloahalang, thepa ea rona e rometsoe linaheng le libakeng tse fetang 10. Re labalabela ho sebelisana le bareki bohle ba tsoang hae le linaheng tse ling. Ho feta moo, khotsofalo ea bareki ke sepheo sa rona sa ka ho sa feleng.
| Mokelikeli oa nomoro ea 1 | |
| Mosebetsi o ikhethileng oa protheine> 800U/mL, mahloriso ke 20mg/mL, ha ho na masala a Nickase | |
| Phofo ea nomoro ea 2 | |
| Ponahalo | Phofo e tšoeu e lyophilized |
| Bohloeki ba electrophoretic | ≥95% |
| Mosebetsi oa li-enzyme | ≥30U/mg |
| Masalla a asiti ea nyutlelie | Ha e bonahale |
| Masalla a Deoxyribonuclease | Ha e bonahale |
| Masalla a Ribonuclease | Ha e bonahale |
| Nomoro ea kariki | G1205-10ML |
| Polokelo | Ha e bolokiloe ho -20 °C, sehlahisoa se boloka ts'ebetso bonyane selemo se le seng, se tsamaisoa ka mokotla oa leqhoa. |
Protheine Kke protease ea serine e leng ea lelapa la subtilisin e nang le ts'ebetso e sebetsang ea enzymatic le ho ikhetha ha substrate ka bophara, e khethollang ka ho khetheha libonto tsa ester le li-peptide tse haufi le C-terminus ea li-amino acid tse hydrophobic, li-amino acid tse nang le sebabole, le libonto tsa amino acid tse nkhang hamonate, tse atisang ho sebelisoa ho senya liprotheine ho hlahisa li-peptide tse khutšoane. Proteinase K e na le tšobotsi ea triad e tloaelehileng ea catalytic Asp39-His69-Ser224 e ikhethang ho li-protease tsa serine 'me e na le libaka tse peli tsa Ca2+ tse tlamang ho potoloha setsi se sebetsang ho eketsa botsitso ba sona le ho boloka ts'ebetso e phahameng ea enzymatic tlas'a maemo a mangata.
1. Sete ea tlhahlobo ea liphatsa tsa lefutso.
2. Lisebelisoa tsa ho ntša RNA le DNA.
3. Ntša likarolo tse seng tsa protheine liseleng 'me u senye litšila tsa protheine (joalo ka ho lokisetsa liente tsa DNA le heparin).
4. Tokisetso ea DNA ea Chromosomal bakeng sa Pulse Electrophoresis.
5. Ho hlakola ka bophirima.
6. Ntlafatso le tlhahiso ea li-reagent tsa albin tse nang le enzyme glycated lefapheng la tlhahlobo ea in vitro.
1. 30mg/botlolo 1g/botlolo kapa 100g/botlolo
2. 1kgs/mokotlana oa foil
Proteinase K ke protease e tsitsitseng ea serine e nang le ho khetheha ho pharaletseng ha substrate. E senya liprotheine tse ngata sebakeng sa tlhaho esita le ha ho na le sesepa. Ha e kenngoe tšebetsong ke li-ion tsa tšepe, li-chelating agents (mohlala, EDTA), li-reagent tsa sulfhydryl, kapa ke li-tripsin kapa li-inhibitor tsa chymotrypsin. E tsitsitse holim'a pH e pharaletseng (4-12.5), ka ts'ebetso e ntle ka ho fetisisa ho pH 6.5-9.5. Ts'ebetso e ka hlohlelletsoa ka ho eketsa li-denaturing agents (SDS le Urea). Denaturation e potlakileng ea enzyme e etsahala mochesong o kaholimo ho 70°C. Autolysis ea enzyme e etsahala ka bongata ho alkaline pH. Leha ho le joalo, Proteinase K ha e kenngoe tšebetsong ka botlalo ke autolysis. Likaroloana tse ling tsa enzyme li tsoela pele ho boloka ts'ebetso ea tsona e felletseng ea proteolytic, esita le ka mor'a autolysis e kholo. Proteinase K e sebelisoa khafetsa lits'ebetsong tsa baeloji ea limolek'hule ho sila liprotheine tse sa batleheng, joalo ka li-nucleases litokisetsong tsa DNA kapa RNA tse tsoang ho likokoana-hloko, lisele tse lengoang le limela. Hangata enzyme ena e sebediswa ho 50–200 ug/ml ditokisetsong tsa nucleic acid ho pH 7.5–8.0 le 37~55oC. Dinako tsa ho futhumala di fapana ho tloha metsotsong e 30 ho isa ho dihora tse 18.
Protease ea serine e tsitsitseng le e arabelang haholo; e sebelisetsoa ho arola protheine le nucleic acid. Proteinase K e sebelisoa ho hloekiseng RNA le DNA ho tsoa liseleng kapa mela ea sele.
Re ikemiselitse ho fana ka theko e phahameng ea tlholisano, lihlahisoa le litharollo tse ikhethang, hammoho le phano e potlakileng bakeng sa Alkaline ProteinaseK ea fektheri e theko e tlase.CAS39450-01-6Re ntse re labalabela ka tieo ho sebelisana le bareki lefatšeng ka bophara. Re lumela hore re tla u khotsofatsa. Re boetse re amohela bareki ka mofuthu ho ea mokhatlong oa rona le ho reka lihlahisoa tsa rona.
theko e tlase ea fektheriProteinase ea Alkaline ea Chaena le 39450-01-6, Ka lebaka la boleng bo botle le litheko tse utloahalang, thepa ea rona e rometsoe linaheng le libakeng tse fetang 10. Re labalabela ho sebelisana le bareki bohle ba tsoang hae le linaheng tse ling. Ho feta moo, khotsofalo ea bareki ke sepheo sa rona sa ka ho sa feleng.
