Protheine K CAS 39450-01-6 e rekisoang haholo fekthering e tsebahalang lefatšeng ka bophara ka sekhahla se phahameng sa ho pepesehela le letsatsi la morao-rao la tlhahiso
Setho se seng le se seng se tsoang sehlopheng sa rona se seholo sa lekeno la ts'ebetso se ananela litlhoko tsa bareki le puisano ea mokhatlo bakeng sa Feme e rekisoang haholo Lefatšeng ka BopharaProtheine K CAS 39450-01-6Ka Sekhahla se Phahameng sa Tlhahiso le Letsatsi la Morao-rao la Tlhahiso, Hona joale, re labalabela tšebelisano-'moho e kholoanyane le bareki ba mose ho maoatle ho latela melemo e kopanetsoeng. Etsa bonnete ba hore ha u ikutloe u le litšenyehelo ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi.
Setho se seng le se seng se tsoang sehlopheng sa rona se seholo sa lekeno la ts'ebetso se ananela litlhoko tsa bareki le puisano ea mokhatlo bakeng saChaena 39450-01-6, Re amohela tšehetso ea hau ka mofuthu 'me re tla sebeletsa bareki ba rona lapeng le kantle ho naha ka thepa ea boleng bo holimo le tšebeletso e ntle e shebaneng le mokhoa oa nts'etsopele e tsoelang pele joalo ka mehla. Re lumela hore o tla rua molemo ho botsebi ba rona haufinyane.
| Mokelikeli oa nomoro ea 1 | |
| Mosebetsi o ikhethileng oa protheine> 800U/mL, mahloriso ke 20mg/mL, ha ho na masala a Nickase | |
| Phofo ea nomoro ea 2 | |
| Ponahalo | Phofo e tšoeu e lyophilized |
| Bohloeki ba electrophoretic | ≥95% |
| Mosebetsi oa li-enzyme | ≥30U/mg |
| Masalla a asiti ea nyutlelie | Ha e bonahale |
| Masalla a Deoxyribonuclease | Ha e bonahale |
| Masalla a Ribonuclease | Ha e bonahale |
| Nomoro ea kariki | G1205-10ML |
| Polokelo | Ha e bolokiloe ho -20 °C, sehlahisoa se boloka ts'ebetso bonyane selemo se le seng, se tsamaisoa ka mokotla oa leqhoa. |
Protheine Kke protease ea serine e leng ea lelapa la subtilisin e nang le ts'ebetso e sebetsang ea enzymatic le ho khetheha ha substrate ka bophara, e khethollang ka ho khetheha libonto tsa ester le li-peptide tse haufi le C-terminus ea li-amino acid tse hydrophobic, li-amino acid tse nang le sebabole, le libonto tsa amino acid tse nkhang hamonate, tse atisang ho sebelisoa ho senya liprotheine ho hlahisa li-peptide tse khutšoane.Protheine Ke na le tšobotsi ea triad e tloaelehileng ea catalytic Asp39-His69-Ser224 e ikhethang ho li-protease tsa serine 'me e na le libaka tse peli tsa Ca2+ tse tlamang ho potoloha setsi se sebetsang ho eketsa botsitso ba eona le ho boloka ts'ebetso e phahameng ea enzymatic tlas'a maemo a mangata.
1. Sete ea tlhahlobo ea liphatsa tsa lefutso.
2. Lisebelisoa tsa ho ntša RNA le DNA.
3. Ntša likarolo tse seng tsa protheine liseleng 'me u senye litšila tsa protheine (joalo ka ho lokisetsa liente tsa DNA le heparin).
4. Tokisetso ea DNA ea Chromosomal bakeng sa Pulse Electrophoresis.
5. Ho hlakola ka bophirima.
6. Ntlafatso le tlhahiso ea li-reagent tsa albin tse nang le enzyme glycated lefapheng la tlhahlobo ea in vitro.
1. 30mg/botlolo 1g/botlolo kapa 100g/botlolo
2. 1kgs/mokotlana oa foil
Proteinase K ke protease e tsitsitseng ea serine e nang le ho khetheha ho pharaletseng ha substrate. E senya liprotheine tse ngata sebakeng sa tlhaho esita le ha ho na le sesepa. Ha e kenngoe tšebetsong ke li-ion tsa tšepe, li-chelating agents (mohlala, EDTA), li-reagent tsa sulfhydryl, kapa ke li-tripsin kapa li-inhibitor tsa chymotrypsin. E tsitsitse holim'a pH e pharaletseng (4-12.5), ka ts'ebetso e ntle ka ho fetisisa ho pH 6.5-9.5. Ts'ebetso e ka hlohlelletsoa ka ho eketsa li-denaturing agents (SDS le Urea). Denaturation e potlakileng ea enzyme e etsahala mochesong o kaholimo ho 70°C. Autolysis ea enzyme e etsahala ka bongata ho alkaline pH. Leha ho le joalo, Proteinase K ha e kenngoe tšebetsong ka botlalo ke autolysis. Likaroloana tse ling tsa enzyme li tsoela pele ho boloka ts'ebetso ea tsona e felletseng ea proteolytic, esita le ka mor'a autolysis e kholo. Proteinase K e sebelisoa khafetsa lits'ebetsong tsa baeloji ea limolek'hule ho sila liprotheine tse sa batleheng, joalo ka li-nucleases litokisetsong tsa DNA kapa RNA tse tsoang ho likokoana-hloko, lisele tse lengoang le limela. Hangata enzyme ena e sebediswa ho 50–200 ug/ml ditokisetsong tsa nucleic acid ho pH 7.5–8.0 le 37~55oC. Dinako tsa ho futhumala di fapana ho tloha metsotsong e 30 ho isa ho dihora tse 18.
Protease ea serine e tsitsitseng le e arabelang haholo; e sebelisetsoa ho arola protheine le nucleic acid. Proteinase K e sebelisoa ho hloekiseng RNA le DNA ho tsoa liseleng kapa mela ea sele.
Setho se seng le se seng se tsoang sehlopheng sa rona se seholo sa lekeno la ts'ebetso se ananela litlhoko tsa bareki le puisano ea mokhatlo bakeng sa fektheri e rekisoang haholo Lefatšeng ka bophara Proteinase K CAS39450-01-6Ka Sekhahla se Phahameng sa Tlhahiso le Letsatsi la Morao-rao la Tlhahiso, Hona joale, re labalabela tšebelisano-'moho e kholoanyane le bareki ba mose ho maoatle ho latela melemo e kopanetsoeng. Etsa bonnete ba hore ha u ikutloe u le litšenyehelo ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi.
Feme e rekisoang haholoChaena 39450-01-6， Re amohela tšehetso ea hau ka mofuthu 'me re tla sebeletsa bareki ba rona lapeng le kantle ho naha ka thepa ea boleng bo holimo le tšebeletso e ntle e shebaneng le mokhoa oa nts'etsopele e tsoelang pele joalo ka mehla. Re lumela hore o tla rua molemo ho botsebi ba rona haufinyane.
