Fektheri e rekisoang ka ho fetesisa ea Proteinase K CAS 39450-01-6 e nang le sekhahla se phahameng sa ho pepeseha le letsatsi la morao-rao la tlhahiso.
Setho se seng le se seng se tsoang ho sehlopha sa rona se seholo sa lekeno la tšebetso se ananela litlhoko tsa bareki le puisano ea mokhatlo bakeng sa Fektheri e rekisoang ka ho fetisisa lefatšeng ka bophara.Proteinase K CAS 39450-01-6ka Sekhahla se Phahameng sa Ho pepeseha le Letsatsi la Morao-rao la Tlhahiso, Hajoale, re lebelletse tšebelisano-'moho e kholoanyane le bareki ba mose ho maoatle ho ipapisitsoe le melemo e kopaneng. Etsa bonnete ba hore ha u na litšenyehelo tsa ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi.
Setho se seng le se seng se tsoang ho sehlopha sa rona se seholo sa lekeno la ts'ebetso se ananela litlhoko tsa bareki le puisano ea mokhatlo bakeng saChina 39450-01-6, Re amohela tšehetso ea hau ka mofuthu 'me re tla sebeletsa bareki ba rona lapeng le mose ho maoatle ka thepa ea boleng bo holimo le ts'ebeletso e ntle e reretsoeng tsoelo-pele e tsoelang pele joalo ka kamehla. Re lumela hore u tla rua molemo ka botsebi ba rona haufinyane.
| No.1 mokelikeli | |
| Ts'ebetso e khethehileng ea protheine> 800U/mL, khatello ea maikutlo ke 20mg/mL, ha ho na masala a Nickase. | |
| No.2 phofo | |
| Ponahalo | White lyophilized phofo |
| Ho hloeka ha electrophoretic | ≥95% |
| Ts'ebetso ea enzyme | ≥30U/mg |
| Nucleic acid mesaletsa | E sa bonahaleng |
| Masala a Deoxyribonuclease | E sa bonahaleng |
| Masala a ribonuclease | E sa bonahaleng |
| Koloi No. | G1205-10ML |
| Polokelo | Ha e bolokiloe ho -20 ° C, sehlahisoa se boloka ts'ebetso bonyane lilemo tse 1, lipalangoang tsa mokotla oa leqhoa. |
Proteinase Kke serine protease ea lelapa la subtilisin e nang le ts'ebetso e sebetsang ea enzymatic le sebopeho se pharaletseng sa substrate, eo ka ho khetheha e senyang litlamo tsa ester le li-peptide tse haufi le C-terminus ea hydrophobic amino acid, li-amino acid tse nang le sebabole, le litlamo tse nkhang hamonate tsa amino acid. hangata e sebelisoa ho theola liprotheine ho hlahisa li-peptide tse khutšoane. Proteinase K e na le tšobotsi e tloaelehileng ea catalytic triad Asp39-His69-Ser224 e ikhethang ho serine proteases 'me e na le libaka tse peli tse tlamang tsa Ca2+ ho potoloha setsi se sebetsang ho eketsa botsitso le ho boloka ts'ebetso e phahameng ea enzymatic tlas'a maemo a mangata a mangata.
1. Setsi sa tlhahlobo ea liphatsa tsa lefutso.
2. RNA le DNA extraction kits.
3. Ntša likaroloana tse se nang protheine ka har'a lisele le ho senya litšila tsa protheine (joaloka ho lokisa liente tsa DNA le heparin).
4. Ho lokisetsa DNA ea Chromosomal bakeng sa Pulse Electrophoresis.
5. Ho phunya ha Bophirimela.
6. Nts'etsopele le tlhahiso ea li-reagents tsa albin tsa enzymatic glycated tšimong ea tlhahlobo ea in vitro.
1. 30mg/botlolo 1g/botlolo kapa 100g/botlolo
2. 1kgs / mokotla oa foil
Proteinase K ke serine protease e tsitsitseng e nang le likarolo tse pharaletseng tsa substrate. E senya liprotheine tse ngata sebakeng sa matsoalloa esita le ka pel'a li-detergents. Ha e kenngoe ke li-ion tsa tšepe, li-chelating agents (mohlala, EDTA), li-reagents tsa sulfhydryl, kapa ka tripsin kapa chymotrypsin inhibitors. E tsitsitse holim'a mefuta e mengata ea pH (4-12.5), e nang le ts'ebetso e nepahetseng ho pH 6.5-9.5. Ts'ebetso e ka khothaletsoa ka ho eketsoa ha li-agent tsa denaturing (SDS le Urea). Ho phatloha ka potlako ha enzyme ho etsahala mochesong o ka holimo ho 70 ° C. Autolysis ea enzyme e etsahala ka ho eketsehileng ho alkaline pH. Leha ho le joalo, Proteinase K ha e sebetse ka ho feletseng ka autolysis. Likaroloana tse ling tsa enzyme li tsoela pele ho boloka ts'ebetso ea tsona e felletseng ea proteolytic, le kamora ho itlhahloba ho hoholo. Proteinase K e sebelisoa khafetsa lits'ebetsong tsa baeloji ea limolek'hule ho sila liprotheine tse sa batleheng, joalo ka li-nuclease ho DNA kapa litokisetso tsa RNA ho tsoa ho likokoana-hloko, lisele tse tsoetsoeng le limela. Enzyme e atisa ho sebelisoa ho 50-200 ug / ml ka litokisetso tsa nucleic acid ka pH 7.5-8.0 le 37 ~ 55oC. Linako tsa ho incubation li fapana ho tloha metsotso e 30 ho isa ho lihora tse 18.
Serine protease e tsitsitseng le e sebetsang haholo; se sebedisoang bakeng sa protheine le nucleic acid ho itšehla thajana.Proteinase K e sebelisoa ho hloekisoa ha RNA le DNA ho tloha liseleng kapa liseleng.
Setho se seng le se seng se tsoang ho sehlopha sa rona se seholo sa basebetsi se ananela litlhoko tsa bareki le puisano ea mokhatlo bakeng sa Fektheri e rekisoang ka ho fetesisa ea Proteinase e Ratoang Lefatšeng ka bophara K CAS39450-01-6ka Sekhahla se Phahameng sa Ho pepeseha le Letsatsi la Morao-rao la Tlhahiso, Hajoale, re lebelletse tšebelisano-'moho e kholoanyane le bareki ba mose ho maoatle ho ipapisitsoe le melemo e kopaneng. Etsa bonnete ba hore ha u na litšenyehelo tsa ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi.
Feme e rekisoang ka ho fetesisaChina 39450-01-6, Re amohela tšehetso ea hau ka mofuthu 'me re tla sebeletsa bareki ba rona lapeng le mose ho maoatle ka thepa ea boleng bo holimo le ts'ebeletso e ntle e reretsoeng tsoelo-pele e tsoelang pele joalo ka kamehla. Re lumela hore u tla rua molemo ka botsebi ba rona haufinyane.
